MAP9 Exhibits Protumor Activities and Immune Escape toward Bladder Cancer by Mediating TGF-β1 Pathway

Figure 7

MAP9 regulates growth 1/synthesis (G1/S) transition-related genes. (a) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was conducted to measure the levels of the genes cyclin D1, cyclin E1, cyclin-dependent kinase 2 (CDK2), CDK4, CDK6, CDK inhibitor 2B (CDKN2B; also named p15), CDKN1A (also named p21), CDKN1C (also named p57), cell division cycle 25 A (CDC25A; also named p16), retinoblastoma transcriptional corepressor 1 (Rb1), and E2F transcription factor (E2F1) in the sh-ctrl (control group) and short hairpin ribonucleic acid- (RNA-) MAP9 (sh-MAP9) MAP9 cells. (b) Western blotting was conducted to measure cyclin D1, cyclin E1, CDK2, CDK4, CDK6, CDKN2B, CDKN1A, CDKN1C, CDC25A, E2F1, and pRb1 protein levels in the sh-ctrl and sh-MAP9 T24 cells. Data are presented as the (SD), and paired Student’s -test was employed for data analysis. , , and .

(a)

(b)