Curcumol Targeting PAX8 Inhibits Ovarian Cancer Cell Migration and Invasion and Increases Chemotherapy Sensitivity of Niraparib

Figure 3

Knockdown of PAX8 inhibits the proliferation, invasion and EMT of ovarian cancer. (a). Verification of the efficiency of knocking down shRNA PAX8 in IGROV-1 and OVCAR-3 cells. (b). CCK-8 experiment verified that knocking down PAX8 inhibits the proliferation of ovarian cancer cells IGROV-1 and OVCAR-3. (c). Knockdown of PAX8 inhibits the migration ability of ovarian cancer cells IGROV-1 and OVCAR-3. (d). Knockdown of PAX8 inhibits the invasion of ovarian cancer cells IGROV-1 and OVCAR-3 by transwell assay. (e). After knocking down PAX8, detection of Snail1 expression in IGROV-1 and OVCAR-3 cells. (f). After knocking down PAX8, detection of Twist1 expression in IGROV-1 and OVCAR-3 cells. (g). Detection of Zeb2 expression in IGROV-1 and OVCAR-3 cells after knocking down PAX8. (h). After knocking down PAX8, detection of MMP13 expression in IGROV-1 and OVCAR-3 cells. (i). After knocking down PAX8, detection of E-cadherin expression in IGROV-1 and OVCAR-3 cells. (j). After knocking down PAX8, detection of Vimentin expression in IGROV-1 and OVCAR-3 cells. P <0.05, P <0.01, vs sh-NC group.

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