Acidic Tumor Microenvironment Promotes Pancreatic Cancer through miR-451a/MEF2D Axis

Figure 4

MEF2D is a target gene of miR-451a. (a) The target genes were predicted by ENCORI, miRWalk, and TargetScan. (b) The miR-451a putative binding sites and corresponding mutant sites of MEF2D. (c) Luciferase reported assay. (d) The mRNA expression of MEF2D detected by qRT-PCR in miR-451a mimics-treated PANC-1 cells. (e) The protein expression of MEF2D in miR-451a mimics-treated PANC-1 cells. (f) The protein expression of MEF2D in PANC-1 cells exposed to acidic conditions. Error bars, mean ± SD from three independent experiments.  < 0.01;  < 0.001.

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