IL-2 Combined with IL-15 Enhanced the Expression of NKG2D Receptor on Patient Autologous NK Cells to Inhibit Wilms’ Tumor via MAPK Signaling Pathway

Figure 3

The NK cells were detected in WT clinic sample. The proportion of CD3^-CD16⁺CD56⁺ NK in PBMCs were analyzed in WT patients () and normal children () by FACS (a, b). The proportion of CD3^-CD16⁺CD56⁺ NK in WT patient PBMCs with different stage (c). The CD16⁺, CD56⁺ and CD16⁺, and CD56⁺ NK cells were detected in paired WT samples () by immunofluorescence staining (d, e). Cytotoxic cytokines were detected by ELISA in paired WT samples (). Results are shown as . For comparisons, Student’s -test was performed in two group, or one-way analysis of variance (ANOVA) was performed in multiple groups. , , and .

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