IL-2 Combined with IL-15 Enhanced the Expression of NKG2D Receptor on Patient Autologous NK Cells to Inhibit Wilms’ Tumor via MAPK Signaling Pathway

Figure 4

The receptor of NK cell analysis in TCGA dataset and paired WT samples. The gene expression level of activating receptor, inhibitory receptor, and cytokine receptor in NK cells of WT and adjacent kidney in TCGA-WT datasets (a–c). qRT-PCR analyzes the gene expression level of the NKG2D, LAG3 IL-15R, and GGT1 in paired WT sample, respectively (d–g). Quantification analysis of NKG2D protein expression level was performed by Western blot in paired WT sample (h, i). N and T represent nontumor adjacent kidney and WT, respectively. Results are shown as . For comparisons, Student’s -test was performed in two group, or one-way analysis of variance (ANOVA) was performed in multiple groups. , , , and .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)