Glycoprotein M6A Suppresses Lung Adenocarcinoma Progression via Inhibition of the PI3K/AKT Pathway

Figure 3

GPM6A upregulation inhibits the progression of lung adenocarcinoma. (a) GFP expression of A549 and H1299 under transfection with lentivirus packaging with overexpression plasmid (HBLV-h-GPM6A-3xflag-ZsGreen-PURO) (Lv-GPM6A) or control plasmid (HBLV-ZsGreen-PURO) (vector). (b) GPM6A mRNA expression upon transfection with Lv-GPM6A or empty vector. (c) GPM6A protein expression upon transfection with Lv-GPM6A or empty vector. (d) OD value (450 nm) of cells at 0, 24, 48, and 72 h after transfection with Lv-GPM6A or empty vector. (e) Relative number of EdU-positive cells after transfection with Lv-GPM6A or empty vector. (f) Number of colony-forming cells after transfection with Lv-GPM6A or empty vector. (g) Number of migrating cells after transfection with Lv-GPM6A or empty vector. (h) Migration area of cells after transfection with Lv-GPM6A or empty vector. (i) Expression of the EMT markers (E-cadherin, N-cadherin, Zeb1, vimentin, and snail) on WB after transfection with Lv-GPM6A or empty vector. (j) Immunofluorescence expression of EMT markers (E-cadherin, N-cadherin, and vimentin) after transfection with Lv-GPM6A or empty vector. (k) Expression of EMT markers (E-cadherin, N-cadherin, Zeb1, vimentin, and snail) on RT-qPCR after transfection with Lv-GPM6A or empty vector. (l) Tumor volume at different time points in nude mice injected with Lv-GPM6A or vector A549. (m) Tumor weight in nude mice injected with Lv-GPM6A or vector A549. Data shown are the mean ± standard deviation (SD) for three independent experiments. (, , , and ) (GFP: green fluorescence protein).

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