Exosomes Derived from Tumor Cells Initiate Breast Cancer Cell Metastasis and Chemoresistance through a MALAT1-Dependent Mechanism

Figure 2

MALAT1 encapsulated by BC cell-derived Exo facilitates BC cell proliferation, metastasis, and chemoresistance. (a) RT-qPCR detection of MALAT1 expression patterns in MCF-7/ADR cells with MALAT1 silencing. (b) RT-qPCR detection of MALAT1 expression patterns in MCF-7/S cells with MALAT1 overexpression. (c) CCK-8 detection of the viability of MCF-7/ADR cells with MALAT1 silencing. (d) CCK-8 detection of the viability of MCF-7/S cells with MALAT1 overexpression. (e) Scratch assay of migration of MCF-7/ADR cells with MALAT1 silencing. (f) Scratch assay of migration of MCF-7/S cells with MALAT1 overexpression. (g) Transwell assay detection of invasion of MCF-7/ADR cells with MALAT1 silencing. (h) Transwell assay detection of invasion of MCF-7/S cells with MALAT1 overexpression. (i) RT-qPCR detection of MALAT1 mRNA expression patterns in MCF-7/S cells treated with Exo-si-MALAT1 or Exo-oe-MALAT1. (j) CCK-8 detection of the viability of MCF-7/S cells treated with Exo-si-MALAT1 + ADR or Exo-oe-MALAT1 + ADR. (k) Scratch assay of migration of MCF-7/S cells treated with Exo-si-MALAT1 + ADR. (l) Scratch assay of migration of MCF-7/S cells treated with Exo-oe-MALAT1 + ADR. (m) Transwell assay detection of invasion of MCF-7/S cells treated with Exo-si-MALAT1 + ADR. (n) Transwell assay detection of invasion of MCF-7/S cells treated with Exo-oe-MALAT1 + ADR. In panels (a–h), , compared with MCF-7/S cells treated with si-NC or oe-NC. In panel (i) , compared with MCF-7/S cells treated with Exo-si-NC or Exo-oe-NC. In panels j–n, , compared with MCF-7/S cells treated with Exo-oe-NC + ADR or Exo-si-NC + ADR. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were analyzed by unpaired t-test.