Exosomes Derived from Tumor Cells Initiate Breast Cancer Cell Metastasis and Chemoresistance through a MALAT1-Dependent Mechanism

Figure 3

MALAT1 binds to miR-1-3p and consequently decreases its expression in BC cells. (a) Venn diagram of the predicted downstream miRNAs of MALAT1 by the lncACTdb, starBase RAID, and DIANA databases. The four ellipses in the figure represent the prediction results of the four databases, and the central part represents the intersection of the four datasets. (b) RT-qPCR detection of candidate miRNA expression patterns in BC cells. (c) The predicted binding sites between MALAT1 and miR-1-3p, miR-96-5p, miR-101-3p, or miR-1271-5p by the starBase database. (d) Binding between MALAT1 and miR-1-3p confirmed by dual-luciferase reporter gene assay in HEK-293 cells. (e) Enrichment of MALAT1 and miR-1-3p determined by RIP assay. (f) RT-qPCR detection of miR-1-3p expression patterns in MCF-7/ADR cells with silencing of MALAT1. (g) RT-qPCR detection of miR-1-3p expression patterns in MCF-7/S cells overexpressing MALAT1. , compared with MCF-10A, MCF-7/ADR cells treated with si-NC, MCF-7/S cells treated with oe-NC, HEK-293 cells transfected with mimic NC or IgG. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were analyzed by unpaired t-test.