Exosomes Derived from Tumor Cells Initiate Breast Cancer Cell Metastasis and Chemoresistance through a MALAT1-Dependent Mechanism

Figure 6

Silencing of VASP inactivates the Rap1 signaling pathway in BC cells. (a) VASP-related gene interaction network. Each circle represents a gene, and the lines between the circles indicate the correlation between genes. (b) KEGG enrichment analysis of VASP-related genes. The abscissa represents GeneRatio, and the ordinate indicates KEGG entry identifier; each circle represents the number of genes enriched in an entry identifier, and the histogram on the right is the color scale. (c) Western blot analysis of Rap1-GTP/total Rap1 ratio in MCF-7/ADR, MCF-7/S, and MCF-10A cells. , compared with MCF-10A cells. , compared with MCF-7/S cells. (d) RT-qPCR detection of VASP expression patterns in MCF-7/ADR cells with si-VASP. (e) RT-qPCR detection of VASP expression patterns in MCF-7/S cells with overexpression of VASP. (f) Western blot analysis of VASP protein expression patterns in MCF-7/ADR cells with si-VASP. (g) Western blot analysis of VASP protein expression patterns in MCF-7/S cells with overexpression of VASP. (h) Western blot analysis of Rap1-GTP/total Rap1 ratio in MCF-7/ADR cells with si-VASP. (i) Western blot analysis of Rap1-GTP/total Rap1 ratio in MCF-7/S cells with overexpression of VASP. In panels (d), (f), and (h) , compared with MCF-7/ADR treated with si-NC. In panels (e), (g), and (i) , compared with MCF-7/S treated with oe-NC. Data are shown as mean ± standard deviation of three technical replicates.