Exosomes Derived from Tumor Cells Initiate Breast Cancer Cell Metastasis and Chemoresistance through a MALAT1-Dependent Mechanism

Figure 7

Inactivated Rap1 signaling pathway attenuates BC cell proliferation, metastasis, and chemoresistance. (a) Western blot analysis of Rap1-GTP and total Rap1 in the MCF-7/ADR cells treated with ESI-09 and MCF-7/S cells treated with 8-pCPT-2′-O-Me-cAMP. (b) CCK-8 detection of the viability of MCF-7/ADR cells treated with ESI-09 ± ADR. (c) CCK-8 detection of the viability of MCF-7/S cells treated with 8-pCPT-2′-O-Me-cAMP. (d) Scratch assay of migration of MCF-7/ADR cells treated with ESI-09 ± ADR. (e) Scratch assay of migration of MCF-7/S cells treated with 8-pCPT-2′-O-Me-cAMP. (f) Transwell assay detection of invasion of MCF-7/ADR cells treated with ESI-09 ± ADR. (g) Transwell assay detection of invasion of MCF-7/S cells treated with 8-pCPT-2′-O-Me-cAMP. , compared with MCF-7/ADR or MCF-7/S treated with DMSO + ADR. Data are shown as mean ± standard deviation of three technical replicates.