Exosomes Derived from Tumor Cells Initiate Breast Cancer Cell Metastasis and Chemoresistance through a MALAT1-Dependent Mechanism

Figure 8

MALAT1 shuttled by BC cell-derived Exo boosts the tumorigenesis and metastasis of BC cells in vivo by regulating the miR-1-3p/VASP/Rap1 axis. (a) RT-qPCR detection of MALAT1 expression patterns in tumor tissues of mice injected with Lv-MALAT1-transduced MCF-7/ADR. , compared with mice treated with Lv-NC. (b) RT-qPCR detection of MALAT1, miR-1-3p, and VASP expression patterns in tumor tissues of mice treated with Exo-Lv-MALAT1 + ADR. (c) Western blot analysis of VASP expression patterns and Rap1-GTP/total Rap1 ratio in tumor tissues of mice treated with Exo-Lv-MALAT1 + ADR. (d) Quantitative analysis of VASP expression patterns in tumor tissues of mice treated with Exo-Lv-MALAT1 + ADR. (e) Quantitative analysis of Rap1-GTP/total Rap1 ratio in tumor tissues of mice treated with Exo-Lv-MALAT1 + ADR. (f) Representative images showing xenografts in nude mice treated with Exo-Lv-MALAT1 + ADR. (g) Tumor volume of mice treated with Exo-Lv-MALAT1 + ADR at the 0–8th week. (h) Tumor weight of mice treated with Exo-Lv-MALAT1 + ADR at the 8th week. (i) HE staining analysis of liver metastasis and lung metastasis of BC cells in mice treated with Exo-Lv-MALAT1 + ADR. , compared with mice treated with Exo-Lv-NC + ADR. n = 10 for mice following each treatment.