Licoflavone A Suppresses Gastric Cancer Growth and Metastasis by Blocking the VEGFR-2 Signaling Pathway

Figure 3

LA arrests the proliferation of VEGF-stimulated MKN-45 cells. (a) The expression of VEGFR-2 protein in SGC-7901, MKN-45, MGC-803, and GES-1 cells. (b) Relative VEGFR-2 expression levels were quantified in SGC-7901, MKN-45, MGC-803, and GES-1 cells. GSE-1 is the reference. (c) The p-VEGFR-2 expression level of LA-treated VEGF-stimulated MKN-45 cell. (d) Relative p-VEGFR-2 protein expression levels were quantified by normalization to GAPDH values as the mean of three independent experiments. (e) The proliferation of VEGF-stimulated MKN-45 cells was measured by the CCK8 assay. (f) LA inhibited the colony formation of VEGF-stimulated MKN-45 cells. (g) 3D microsphere was observed at 0, 24, 48, 72, and 96 h, respectively.  μm. Values are given as the (SEM) experiments. . vs. control and vs. control. ^# vs. and ^## vs. .

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