Exosome-Mediated miR-4792 Transfer Promotes Bladder Cancer Cell Proliferation via Enhanced FOXC1/c-Myc Signaling and Warburg Effect

Figure 2

Inhibition of c-Myc significantly reversed FOXC1-induced cell growth and aerobic glycolysis in T24 cell lines. T24 cells were transfected with the FOXC1 plasmid. (a) The CCK8 assay was used to measure the number of cells at 0, 12, 24, and 48 h  < 0.01,  < 0.001 vs. vector. (b, c) OCR and ECAR were measured using a Seahorse XF24 analyzer. (d) Lactic acid content was measured. After 24 h of FOXC1 plasmid transfection, 100 μmol/L of c-Myc inhibitor, namely, 10058-F4, was added to the cells. (e) The CCK8 assay was used to measure the number of cells at 0, 12, 24, and 48 h in different groups.  < 0.01,  < 0.001 vs. vector + vehicle; ## < 0.01, ### < 0.001 vs. oeFOXC1 + vehicle. (f, g) OCR and ECAR were measured using a Seahorse XF24 analyzer in different groups. (h) Lactic acid content was measured.  < 0.01 vs. vector + vehicle; ## < 0.01 vs. oeFOXC1 + vehicle.

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