Exosome-Mediated miR-4792 Transfer Promotes Bladder Cancer Cell Proliferation via Enhanced FOXC1/c-Myc Signaling and Warburg Effect

Figure 4

miR-4792 regulation of cell growth and aerobic glycolysis in BLCA cells is probably mediated by modulating the FOXC1 expression. (a–c) miR-4792 inhibitor treatment (antagomir) and agomir mimic transfection of hAMSCs were carried out on 5637 cells. (a, b) miR-4792 levels in hAMSCs. (c) mRNA level of FOXC1 in hAMSCs. (d) Detection of the luciferase activity ratio.  < 0.05,  < 0.01,  < 0.001 vs. NC. (e–g) 5637 cells were untreated or transfected with agomir and empty vector or with agomir and FOXC1 plasmids (oxFOXC1). (e) The CCK8 assay was used to measure the number of cells at 0, 12, 24, and 48 h  < 0.01,  < 0.001 vs. NC; ## < 0.01, ### < 0.001 vs. agomir + vector. (f, g) OCR and ECAR were measured using a Seahorse XF24 analyzer. (h) Lactic acid content was measured.  < 0.01 vs. NC; ### < 0.001 vs. agomir + vector.

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