Prp19 Facilitated p21-Dependent Senescence of Hepatocellular Carcinoma Cells

Figure 2

Inhibiting Prp19 represses senescence of HCC cells. (a) Prp19 expression was decreased by siRNA silencing in HCC cells. (b) Cell cycle distribution of HCC cells with Prp19 knockdown. (c) The effects of Prp19 on HCC cells senescence were assessed using SA-β-galactosidase. (d) The effects of Prp19 on HCC cells senescence were assessed using SA-β-galactosidase staining with or without incubation of 10 μM cisplatin for 24 h. (e) Relative IL6, IL1a, IL1b, IL8, and CXCL1 transcript levels in human HCC cell lines were determined by qRT-PCR. The levels of mRNA were normalized to ACTIN and presented relative to the control cells. Negative control, NC. The data are shown as the mean ± SEM. Similar results were observed in three independent experiments. and . Black scale bar: 100 μm.

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