Posttranslational Modifications of Rev-Erbα Protein and Abnormal Inflammatory Response in Gastric Cancer

Figure 7

Rev-erbα is recruited on the promoters of cytokines, and Nr1d1 gene deletion increases expression of these cytokine genes. (a, b) The expression of Nr1d1, IL-6, TNF-α, VEGF, and IL-10 genes was evaluated by qRT-PCR in Nr1d1 knockout and WT BGC-823 cells. (c) ChIP was performed to detect the recruitment of Rev-erbα on the promoters of IL-6, TNF-α, VEGF, and IL-10 genes in both BGC-823 and SGC-7901 cells. (d) Schematic figure showing that Rev-erbα SUMOylation may cause its protein degradation, and this decreases its recruitment on promoters of cytokine genes, leading to increased glycolysis. Mean ± SEM, N  = 4-5. , vs WT group.

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