Marine-Derived Stichloroside C2 Inhibits Epithelial–Mesenchymal Transition and Induces Apoptosis through the Mitogen-Activated Protein Kinase Signalling Pathway in Triple-Negative Breast Cancer Cells

Figure 2

Stichloroside C2 (STC2) can inhibit the proliferation of breast cancer cells. (a) Three breast cancer cell lines, comprising a human breast cancer cell line (MCF-7), human triple-negative breast cancer (TNBC) cell line (MDA-MB-231), and mouse TNBC cell line (4 T1), and a normal epithelial cell line (IOSE-80) were selected and treated with a series of different concentrations of STC2 after treatment for 24 h, the cell viability was detected using the Cell Counting Kit-8 (CCK-8) method. , , , compared to the control group. (b, c) Inhibition of TNBC cell clone formation by sea cucumber saponin monomer. Human MDA-MB-231 cells and mouse 4 T1 cells were treated with STC2 at different concentrations (0.25, 0.5, and 1 μM) for 24 h. The cells were cultured for 1–2 weeks and stained with crystal violet. The number of clones formed was detected. , , compared to the control group.

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