Autophagy Induced by Muscarinic Acetylcholine Receptor 1 Mediates Migration and Invasion Targeting Atg5 via AMPK/mTOR Pathway in Prostate Cancer

Figure 1

CHRM1 is highly expressed in PCa. (a) Immunohistochemical staining of CHRM1 in subcutaneous tumor-forming tumors of nude mice. Scale bar: PBS: 100 μm. CHRM1: 2 mm. (b) Immunofluorescence detection of CHRM1 in PC-3, LNCaP, and RWPE-1 cells in which blue fluorescence is DAPI and green fluorescence is CHRM1. (c) Western blotting was performed to test CHRM1 expression in the human PCa cell lines PC-3, LNCaP, and DU145, the normal human prostate cell line RWPE-1, and the lung cancer cell line A549. (d) Quantification of the rate of CHRM1/GAPDH in cells. Data represent the mean ± SD of three experiments. ; . (e) Cell crystal violet staining of PC-3, LNCaP, DU145, RWPE-1, and A549.

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