Research Article

HS3ST3A1 and CAPN8 Serve as Immune-Related Biomarkers for Predicting the Prognosis in Thyroid Cancer

Figure 7

The expression of HS3ST3A1 and CAPN8 in clinical samples and cellular functional experiments. (a) GEPIA analysis show HS3ST3A1 and CAPN8 differed among stages. (b) RT-qPCR of HS3ST3A1 and CAPN8 in 20 paired TC tissues, including 10 paired samples with LNM and 10 paired samples with no LNM. (c) IHC staining of HS3ST3A1 and CAPN8 in 30 TC specimens, including 10 adjacent normal samples, 10 tumor samples with no LNM, and 10 tumor samples with LNM. (d) Western blot of downregulation of HS3ST3A1 and CAPN8 in papillary TC cells BPCAP and TPC-1. The effect of HS3ST3A1 and CAPN8 downregulation on proliferation and invasion is determined by (e) MTT, (f) colony formation, and (g) Transwell. , .
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