Research Article
MicroRNA-323a-3p Negatively Regulates NEK6 in Colon Adenocarcinoma Cells
Table 2
The primers for construction of plasmids.
| Name of plasmids | Name of primers | Sequence (5′-3′) |
| Wild-type NEK6 3′ UTR | 3′UTR WT-F | GAGCTCGCTAGCCTCGAGCCGTGCCTTATCAAAGCCAG | 3′UTR WT-R | GCATGCCTGCAGGTCGACGCAGCAGGTGTCAGGAATC | Mutant NEK6 3′ UTR | 3′UTR MUT-F | TGGATTGATTACAGAATCTTTAGGGTAATTC | 3′UTR MUT-R | AAGATTCTGTAATCAATCCAGATACAGGGGGC | pCDH-NEK6 | pCDH-Nflag-F | CTAGAGCTAGCGAATTCGCCACCATGGACTACAAAGACGATGACGAC | Flag-NEK6-F | ACAAAGACGATGACGACAAGATGCCCAGGAGAGAAGTTTG | pCDH-NEK6-R | CTCAGCGGCCGCGGATCCGGTGCTGGACATCCAGATG |
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F: forward primer; R: reverse primer.
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