EGCG Enhances the Chemosensitivity of Colorectal Cancer to Irinotecan through GRP78-MediatedEndoplasmic Reticulum Stress

<div>Effects of EGCG combined with irinotecan on GRP78-mediated ER stress and apoptosis in RKO and HCT116 cells. GRP78 mRNA and protein expression in RKO and HCT116 cells were detected by qPCR and WB after transfection of GRP78 overexpression plasmid (a) or siRNA (b) 24 h. Annexin V-FITC and PI double staining method was employed to determine the effect of GRP78 on drug combination-treated cell apoptosis, and FlowJo software was used to fit the data (c, d). siG-siGRP78, siGAPDH-positive control, siNC-negative siRNA control, pNC-vector control, pGRP78-GRP78 overexpression plasmid. <span class="nowrap"><svg height="10.7395pt" id="M25" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 10.7395" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 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Journal of Oncology

fig5

Figure 5

Figure 5: EGCG Enhances the Chemosensitivity of Colorectal Cancer to Irinotecan through GRP78-MediatedEndoplasmic Reticulum Stress 