Prognostic Value and Potential Regulatory Mechanism of H19 in Stomach Adenocarcinoma

Figure 4

H19 promoted migration, invasion, and survival of STAD cells by binding to five miRNAs. (a) Bioinformatics analysis of 9 miRNAs potentially binding to lncRNA H19. (b) RNA pulldown assay verified the binding of 5 of the 9 miRNAs to lncRNA H19 (n = 3). (c) Relative luciferase activity determined to verify the binding of 5 miRNAs to H19 (n = 3). (d) Scratch assay showing the migration of MKN-45 cells with H19 knockdown or miRNA inhibition (n = 3). (e) Quantitative analysis of the wound closure rate (n = 3). (f) Transwell assays showing the invasion of MKN-45 cells after H19 knockdown or miRNA inhibition (n = 3). (g) Quantitative analysis of the number of migrated cells (n = 3). (h) Flow cytometric analysis of apoptosis induced by gemcitabine in MKN-45 cells transfected with H19 siRNA (n = 3). (i) Quantitative analysis of the percentage of apoptotic cells (n = 3). (j-n) Analysis of potential downstream genes regulated by five miRNAs binding to H19.

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