JPHYD Inhibits miR-21-5p/Smad7-Mediated Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma Cells

Figure 5

JPHYD inhibited the level of miR-21-5p and increased the Smad7 protein expression in MHCC97H and HepG2 cells. (a) After intervention of JPHYD for 24 hours, we observed the expression of miR-21-5p. (b) After transfection of miR-21-5p mimic and inhibitor into the cells, we observed the expression of Smad7. (c) After transfection of mimic, we added JPHYD to intervene for 24 h and observed the expression of Smad7. (d) After transfection by adding overexpressed Smad7 to the cells, PCR experiment was used to detect the expression of miR-21-5p. (e) The dual luciferase verification report experiment further was verified the relationship between miR-21-5p and Smad7. The asterisk () indicates a significant difference from the control ().

(a)

(b)

(c)

(d)

(e)