Neferine Exerts Ferroptosis-Inducing Effect and Antitumor Effect on Thyroid Cancer through Nrf2/HO-1/NQO1 Inhibition

Figure 1

Neferine suppressed the proliferation and enhanced apoptosis of thyroid cancer cells. (a, b) The 2D and 3D structure of neferine. (c) The viability of IHH-4 and CAL-62 cells was detected by the CCK-8 assay after both cells were treated with a series of neferine, including 0, 0.3, 0.6, 1.25, 2.5, 5, 10, 20, 40, and 80 μM for 96 h , and vs. 0 μM. (d) The viability of IHH-4 and CAL-62 cells was examined by the CCK-8 assay after both cells were administrated with 5 and 10 μM neferine for 24, 48, 72, and 96 h vs. control. (e) The apoptosis rate was determined by flow cytometry after IHH-4 and CAL-62 cells were incubated with 5 and 10 μM neferine for 96 h vs. Control. (f–i) The relative protein expression of Ki67, Bax, Bcl-2, and cleaved caspase-3 was measured by western blot assays. The data were expressed after being normalized to β-actin. and vs. control. All assays were performed in triplicate.

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