Long Intergenic Noncoding RNA 00641 Promotes Growth and Invasion of Colorectal Cancer through Regulating miR-450b-5p/GOLPH3 Axis

Figure 6

GOLPH3 overexpression partially reverted the impacts caused by LINC00641 silencing. (a)-(b) qRT-PCR analysis of LINC00641 and GOLPH3 expression in SW480/shNC + Vector, SW480/shLINC00641 + Vector, SW480/shLINC00641 + GOLPH3, HCT-116/shNC + Vector, HCT-116/shLINC00641 + Vector, and HCT-116/shLINC00641 + GOLPH3 cells. (c)Representative image of western blotting GOLPH3 expression in SW480/shNC + Vector, SW480/shLINC00641 + Vector, SW480/shLINC00641 + GOLPH3, HCT-116/shNC + Vector, HCT-116/shLINC00641 + Vector, and HCT-116/shLINC00641 + GOLPH3 cells. (d) MTT arrays indicated that GOLPH3 overexpression reversed the effect of LINC00641 silencing on proliferation. (e)-(f) Flow cytometry analysis showed that GOLPH3 overexpression reversed the effect of LINC00641 silencing on cell cycle and apoptosis. (g)-(h) Transwell assay showed GOLPH3 overexpression reversed the effect of LINC00641 silencing on migration and invasion. Data are represented as mean ± SD of three biological replicates. Compared with SW480/shNC + Vector or HCT-116/shNC + Vector, , , , ns . SW480/shLINC00641 + Vector vs. SW480/shLINC00641 + GOLPH3 OE, HCT-116/shLINC00641 + Vector vs. HCT-116/shLINC00641 + GOLPH3 OE, ^#, ^##, ^###, ^####, ns . Scale bar: 10 μm.

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