Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4⁺CD25⁺FOXP3⁺ Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization

Figure 4

BMSC-derived exosomal miR-181a regulates FOXP3 via SIRT1-mediated acetylation. (a) The binding site of miR-181a and SIRT1 predicted by StarBase. (b) The interaction of SIRT1 and miR-181a determined by a dual-luciferase reporter assay. . (c) Expression of SIRT1 and FOXP3 in CD4⁺ T cells treated with BMSC-derived exosomes detected by Western blot. . (d) Expression of SIRT1 and FOXP3 in CD4⁺ T cells transfected with NC or miR-181a inhibitor BMSC-derived exosomes detected by Western blot. . (e) FOXP3 acetylation in CD4⁺ T cells treated with BMSC-derived exosomes detected by immunoprecipitation. . (f) FOXP3 acetylation in CD4⁺ T cells with CD4⁺ T cells transfected with NC or miR-181a inhibitor and BMSC-derived exosomes detected by immunoprecipitation. . Data are the mean ± SD (n = 3 biological replicates).

(a)

(b)

(c)

(d)

(e)

(f)