Research Article
Bee Venom Triggers Autophagy-Induced Apoptosis in Human Lung Cancer Cells via the mTOR Signaling Pathway
Figure 1
Bee venom induces autophagy in a dose- and time-dependent manner. (a) NCI-H460 cells were treated with bee venom (0.1, 0.5, and 1 μg/ml) for 24 h. LC3 levels were evaluated by immunoblotting. (b) NCI-H460 cells were treated with 1 μg/ml bee venom for the indicated time. LC3 levels were evaluated by immunoblotting. (c) NCI-H460 cells were treated with bee venom (0.1, 0.5, and 1 μg/ml) for 24 h. The formation of LC3 puncta was detected after bee venom treatment under a fluorescence microscope. The percentage of cells along with the number of LC3 puncta was calculated. Scale bar, 10 μm. (d) NCI-H460 cells were treated with 1 μg/ml bee venom for the indicated time. The formation of LC3 puncta was detected after bee venom treatment under a fluorescence microscope. The percentage of cells along with the number of LC3 puncta was calculated. Scale bar, 10 μm. (e) NCI-H460 cells were treated with bee venom (0.1, 0.5, 1, and 5 μg/ml) for 24 h. The indicated protein levels were evaluated by immunoblotting. (f) NCI-H460 cells were treated with 1 μg/ml bee venom for the indicated time. The indicated protein levels were evaluated by immunoblotting.
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