Exosome-Mediated Transfer of miR-3613-5p Enhances Doxorubicin Resistance by Suppression of PTEN Expression in Breast Cancer Cells

Figure 2

The expression of miR-3613-5p is upregulated in exosomes of doxorubicin-resistant breast cancer cells. (a) qRT-PCR was performed to assess the relative level of miR-3613-5p in MCF-7/DOX, MDA-MB-231/DOX, MCF-7, and MDA-MB-231 after the treatments of RNase A and Triton X-100. . Data are of 3 independent experiments. (b) Exosomes were isolated, and the structure was observed by TEM. (c) Western blotting was used to detect the protein expression of TSG101 and CD63 in supernatant and exosomes of MCF-7/DOX and MDA-MB-231/DOX cells. (d) qRT-PCR was performed to assess the relative level of miR-3613-5p in the exosomes of MCF-7, MCF-7/DOX, MDA-MB-231, and MDA-MB-231/DOX. . Data are of 3 independent experiments.

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