Identification and Validation of Three-Gene Signature in Lung Squamous Cell Carcinoma by Integrated Transcriptome and Methylation Analysis

Figure 2

Screening and establishment for DNAm-driven signature associated with prognosis. (a) Survival analysis for CLDN1, ABCC5, and CSTA expressions in LUSC. (b) A 10-foldcross-validation for tuning parameter selection in the Least Absolute Shrinkage and Selection Operator model. Partial likelihood deviance is plotted against log (λ), where λ is the tuning parameter. Dotted vertical lines were drawn at the optimal values by minimum criteria and 1-s.e. criteria. (c) Comparison of OS between the high-risk and low-risk score groups. (d) Time-dependent receiver operating characteristic analysis was performed to evaluate the accuracy of the prognostic signature. (e) Univariate and multivariate regression analysis was performed to identify significantly predictive factors associated with OS. OS, overall survival (days); CLDN1, Claudin 1; ABCC5, ATP-binding cassette subfamily C member 5; CSTA, Cystatin A.

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