Pseudogene MSTO2P Interacts with miR-128-3p to Regulate Coptisine Sensitivity of Non-Small-Cell Lung Cancer (NSCLC) through TGF-β Signaling and VEGFC

Figure 2

MiR-128-3p regulated MSTO2P knockdown-promoted coptisine sensitivity of NSCLC (a) StarBase 2.0 was used to identify miR-128 as target for MSTO2P. (b) RT-qPCR showed miR-128-3p and MSTO2P levels in A549 cells transfected with NC mimic, miR-128-3p mimic, NC inhibitor, miR-128-3p inhibitor. , miR-128-3p mimic vs. NC mimic group, miR-128-3p inhibitor vs. NC inhibitor group. (c) RT-qPCR showed miR-128-3p levels in A549 and Calu-1 cells treated with or without coptisine. , coptisine group vs. mock group. (d) RT-qPCR showed miR-128-3p levels in A549 cells transfected with si-NC, si-MSTO2P, si-MSTO2P plus miR-128-3p inhibitor. , si-MSTO2P group vs. si-NC group, si-MSTO2P + miR-128-3p inhibitor group vs. si-MSTO2P group. (e) Cell viability was determined by MTT in A549 cells transfected with si-NC, si-MSTO2P, si-MSTO2P plus miR-128-3p inhibitor under coptisine treatment. , , si-MSTO2P group vs. si-NC group, si-MSTO2P + miR-128-3p inhibitor group vs. si-MSTO2P group. (f and g) Cell apoptosis was determined by TUNEL in A549 cells transfected with si-NC, si-MSTO2P, si-MSTO2P plus miR-128-3p inhibitor under coptisine treatment. , si-MSTO2P group vs. si-NC group, si-MSTO2P + miR-128-3p inhibitor group vs. si-MSTO2P group. Scale bar = 5 μm. (h and i) Cell invasion was determined in A549 cells transfected with si-NC, si-MSTO2P, si-MSTO2P plus miR-128-3p inhibitor under coptisine treatment. , , si-MSTO2P group vs. si-NC group, si-MSTO2P + miR-128-3p inhibitor group vs. si-MSTO2P group. Scale bar = 5 μm.

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