Pseudogene MSTO2P Interacts with miR-128-3p to Regulate Coptisine Sensitivity of Non-Small-Cell Lung Cancer (NSCLC) through TGF-β Signaling and VEGFC

Figure 4

VEGFC was a downstream effector for MSTO2P/miR-128-3p in NSCLC cells (a) VEGFC was identified as target of miR-128-3p by TargetScan. (b) VEGFC levels were determined by RT-qPCR in A549 cells transfected with NC mimic, miR-128-3p mimic, miR-128-3p mimic plus pcDNA3.1-VEGFC. , miR-128-3p mimic group vs. NC mimic group, miR-128-3p mimic + VEGFC group vs. miR-128-3p mimic group. (c) Cell viability was determined by MTT in A549 cells transfected with NC mimic, miR-128-3p mimic, miR-128-3p mimic plus pcDNA3.1-VEGFC under coptisine treatment. , , miR-128-3p mimic group vs. NC mimic group, miR-128-3p mimic + VEGFC group vs. miR-128-3p mimic group. (d and e) Cell apoptosis was determined by TUNEL in A549 cells transfected with NC mimic, miR-128-3p mimic, miR-128-3p mimic plus pcDNA3.1-VEGFC under coptisine treatment. , miR-128-3p mimic group vs. NC mimic group, miR-128-3p mimic + VEGFC group vs. miR-128-3p mimic group. Scale bar = 5 μm. (f and g) Cell invasion was determined in A549 cells transfected with NC mimic, miR-128-3p mimic, miR-128-3p mimic plus pcDNA3.1-VEGFC under coptisine treatment. , miR-128-3p mimic group vs. NC mimic group, miR-128-3p mimic + VEGFC group vs. miR-128-3p mimic group. Scale bar = 5 μm. (h) The working model shows regulation of NSCLC response to coptisine by MSTO2P.

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