Polypeptide N-Acetylgalactosaminyltransferase 14 (GALNT14) as a Chemosensitivity-Related Biomarker for Osteosarcoma

Figure 6

Interfering with GALNT14 expression promoted the inhibitory effect of cisplatin and doxorubicin on the proliferation of osteosarcoma cell lines. (a) The expression of GALNT14 in normal human osteoblasts, drug-sensitive osteosarcoma, and drug-resistant osteosarcoma. (b) GALNT14 siRNA transfection in osteosarcoma cell lines MG-63 and U-2. (c) MG-63 and U-2 OS cells were treated with Cis (20 μmol/L) or Dox (0.2 μg/mL) for 24 h. Then, cell viability was determined at 24 h, 48 h, and 72 h by the CCK-8 assay. (d) MG-63 or U-2 OS cells were seeded in six-well plates and treated with Cis (5 μmol/L) and Dox (0.05 μg/mL). The plates were imaged at 14 days to assess clones. Cis, cisplatin; Dox, doxorubicin. , , , .

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