Suppression of GCH1 Sensitizes Ovarian Cancer and Breast Cancer to PARP Inhibitor

Figure 5

GCH1 was associated with homologous recombination repair (HRR) pathway. GSEA analysis of differentially expressed genes between high- and low-GCH1 expression groups showing the enrichment of homologous recombination repair (HRR) pathway in (a) breast cancer and (b) ovarian cancer. (c-d) Shown are expression profiles of 28 HRR-related genes in high- and low-GCH1 groups, and data are presented by heatmaps. Correlation diagrams showing the association between GCH1 expression and HRR-related genes, including FANCA and BRCA2 in (e) breast cancer and RAD54L and FANCI in (f) ovarian cancer. Representative immunofluorescence images and quantification of γ-H2AX foci and Rad51 foci formation in control and 5 mM DAHP-treated groups of (g, i) MDA-MB-231 cells and (h, j) A2780 cells. 10 μM niraparib was used as positive control and all the drugs were administered for 24 hours.

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