METTL14 Regulates PLAGL2/β-Catenin Signaling Axis to Promote the Development of Nonsmall Cell Lung Cancer

Figure 2

PLAGL2 activated β-catenin signaling to influence cell proliferation and migration. (a) β-catenin (total) and β-catenin (nuclear) protein expressions in NSCLC tissues. ^∗ < 0.05 vs. paracarcinoma tissues. (b) PLAGL2 and β-catenin mRNA expressions. (c) β-catenin (total) and β-catenin (nuclear) protein expressions in cells. (d) IF detection of β-catenin localization. ^∗ < 0.05 vs. NC. (e) PLAGL2 and β-catenin mRNA expressions. (f) Plate clone formation assay to evaluate cell proliferation. (g) Cell migration was tested by wound healing assay. Student’s t-test was used between the two groups. Comparisons among multiple groups were conducted by one-way analysis of variance (ANOVA), followed by Tukey’s post hoc test. ^∗ < 0.05 vs sh-NC, ^# < 0.05 vs sh-PLAGL2 + oe-NC.

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