The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses

Figure 4

IMPDH1 was able to inhibit the cell growth and migration of HCCC-9810. (a and b). The level of mRNA and protein of IMPDH1. (c) The cloning ability of HCCC-9810. (d) The apoptosis rate of HCCC-9810 analyzed by flow cytometry. (e) Migration of cell number of HCCC-9810. (f) HCCC-9810 invasion was detected by transwell. (g) Western blot was used to detect E-cadherin, N-cadherin, and vimentin. (h) The level of protein of cell markers CD133 and SOX2.  < 0.05 compared with the si-NC group. The data are expressed as mean ± SD. Comparisons among multiple groups were analyzed using one-way ANOVA.

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