Deciphering the Molecular Mechanism of Yifei-Sanjie Pill in Cancer-Related Fatigue

Figure 4

YFSJ alleviates CRF by inhibiting mitophagy induced by the Stat3/HIF-1α/BNIP3 signaling pathway overactivation in skeletal muscle. (a) The IHC staining and their relative expression levels were performed to observe proteins associated with autophagy and inflammation in skeletal muscle tissues of mice from each group. (b) WB analysis was performed to determine the protein expression of the Stat3/HIF-1α/BNIP3 signaling pathway and mitophagy-related proteins and (c, d) their relative expression levels. GAPDH or COX IV was used as the internal control. Data were presented as mean ± SD; ^ns , , , and compared to the control group; ^ns#, ^#, ^##, and ^### compared to the CRF group; ^ns&, ^&, and ^&& compared to the low group; n = 3.

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