Deciphering the Molecular Mechanism of Yifei-Sanjie Pill in Cancer-Related Fatigue

Figure 6

Quercetin can reverse TNF-α-induced mitophagy in C2C12 myoblasts. (a–d) Effect of IL-6, TNF-α, YFSJ, and quercetin on the viability of C2C12 myoblasts, respectively, n = 3. (e) Reverse effect of YFSJ (2 mg/ml), quercetin (1.5 μM), and 3-MA (pretreatment with 2 mM for 4 h) on TNF-α (20 ng/ml)-induced decline viability and (f) increase autophagy in C2C12 myoblast, n = 3. (g) WB analysis was performed to determine the protein expression of the Stat3/HIF-1α/BNIP3 signaling pathway and mitophagy related proteins, and (h, i) their relative expression levels. GAPDH or COX IV was used as the internal control. Data were presented as mean ± SD; ^ns, , and compared with the blank group; ^ns#, ^##, and ^###1 compared with the TNF-α group; n = 3.

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