Research Article
miR-4757-3p Inhibited the Migration and Invasion of Lung Cancer Cell via Targeting Wnt Signaling Pathway
Figure 4
Wnt5a or Wnt8b silencing regulated the migration and invasion in. A459 cells and mediated the activation of the Wnt signaling pathway. (a, b) Real-time PCR analysis of Wnt5a and Wnt8b expressions in A549 cells. (c, d) Western blot and real-time PCR analysis of Wnt5a and Wnt8b expressions in A549 cell transfection with Wnt5a-siRNA or Wnt8b-siRNA. (e) CCK8 analysis of cell viability in A549 cell transfection with Wnt5a-siRNA or Wnt8b-siRNA. (f) Transwell analysis of cell invasion and migration in A549 cell transfection with Wnt5a-siRNA or Wnt8b-siRNA. (g) Real-time PCR analysis of β-catenin expression in A549 cell transfection with Wnt5a-siRNA or Wnt8b-siRNA. .
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