Microdissecting the Hypoxia Landscape in Colon Cancer Reveals Three Distinct Subtypes and Their Potential Mechanism to Facilitate the Development of Cancer

Figure 7

Verification of the mRNA expression levels of the five key genes in HRI signature by qRT-PCR. (a) Heatmap of the mRNA expression levels of all eleven genes in HRI signature in TCGA-COAD cohort. Comparison of relative mRNA expression levels of RGS16 (b), SNAI1 (c), CDR2L (d), FRMD5 (e), and FSTL3 (f) between 30 pairs of CC tumor and adjacent normal tissues using the qRT-PCR experiment. HRI: hypoxia-related index. qRT-PCR: quantitative reverse transcription polymerase chain reaction. N: normal; T: tumor; CC: colon cancer.

(a)

(b)

(c)

(d)

(e)

(f)