Journal of Obesity / 2010 / Article / Tab 2 / Research Article
Feeding Regulates the Expression of Pancreatic Genes in Gastric Mucosa Table 2 Transcripts with defence/protection roles and other genes differentially modulated by feeding in the gastric mucosa.
Tag Fasting Low fat High fat Description 30 min 1 h 3 h 30 min 1 h 3 h Cellular defence/ CCAGGCCTTAC 53 23 44 11 ↓ 44 47 32 Cysteine-rich protein 1 (Mm.272368; NM_007763) TGTTCAGTTTT 0 2 23 ↑ 0 0 8 0 Heat shock protein 1A-Hsp70 1A (Mm.6388; BC054782) TCTACACTGCC 0 15 56 ↑ 7 10 45 ↑ 0 EST Heat shock protein 1B-Hsp70 1B (Mm.372314; BP766094) TAACTGACAAT 91 38 37 26 ↓ 64 66 43 Metallothionein 2 (Mm.147226; BC031758)/EST Phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha-Pip5k1a (Mm.296409; BU744153) AACGCTTTCTA 81 27 46 33 34 45 11 ↓ Placenta-specific 8 (onzin) (Mm.34609; BC010789) CAGGAGGAGTT 28 13 21 18 22 4 41
*
Protein disulfide isomerase associated 3 (Mm.263177; BC033439) GTGTGCGCTGG 10 0 0 38 6 0 0
*
EST Elastase 2A (Neutrophil elastase) (Mm.45316; BM730866) TCGCTGCTTTA 75 44 40 11 ↓ 24 23 25 EST WD repeat domain 92 (Mm.298132; CO039767)
Regulatory mechanisms TATTTCAGTGA 0 6 8 27 ↑ 12 6 5 Adenylate cyclase 6 (Mm.157091; NM_007405) GGCTGTCCTGT 0 2 15 13 2 6 25 ↑ DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (Mm.29644; BC096036) TCCTGAAGAGG 2 8 31 ↑ 13 12 16 16 Furin (paired basic amino acid cleaving enzyme) (Mm.5241; BC048234) AATTTCTTCCT 24 67 12 26 8
*
18 2 Major urinary protein 1/2 (Mm.335875; BC099597); (Mm.457981; AC:BC012259). CAGCAAATGAA 6 6 0 33 4 2 0
*
EST Kallikrein 1 (Mm.142722; BG871914) Others AAAAATCATCG 22 4 67 62 62 76 93 ↑ NADH dehydrogenase 5, mt-Nd5 (CDNA clone IMAGE: 4910858; Mm.455357; BC055066) GACCTGGAGCC 30 23 8 2 ↓ 14 6 7 EST Ribosomal protein S14 (Mm.43778; AV212419)
Numbers indicate the count of serial analysis of gene expression (SAGE) tags per 50000.↓ indicate a significant differential regulation by LF or HF feeding compared to fasting (
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