104706.fig.004a
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104706.fig.004b
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104706.fig.004c
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104706.fig.004d
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Figure 4: Dose effect of 5, 15, and 25 μg/mL of thyme extracts S1 and S2 from three different species (Thymus vulgaris, Thymus zygis, and Thymus hyemalis) on the production and secretion of TNF-α (a), IL-1β (b), IL-6 (c), and IL-10 (d) on macrophage-THP-1 cells. Cells were differentiated with (PMA) 100 ng/mL for 24 h and afterwards treated as described in Section 3 for 48 hours. Data represent means ± SEM calculated from six independent experiments with 3 replications for each treatment. Statistic Dunnett's multiple comparison test versus Ctrl +oxLDL signification is represented by * 𝑃 values less than 0.05, significant; ** 𝑃 values less than 0.01, very significant; and *** 𝑃 values less than 0.001, extremely significant. One group was treated with a small concentration of diclofenac (5 μg/mL) as positive standard control. Statistic Bonferroni multiple comparison test versus Ctrl −oxLDL signification is represented by ♦♦ 𝑃 values less than 0.01, very significant; and ♦♦♦ 𝑃 values less than 0.001, extremely significant. One group was treated with a small concentration of diclofenac (5 μg/mL) as positive standard control.