Cardiomyocyte Triglyceride Accumulation and Reduced Ventricular Function in Mice with Obesity Reflect Increased Long Chain Fatty Acid Uptake and De Novo Fatty Acid Synthesis
Figure 5
(a) Cardiac gene expression ratios for cardiac LCFA transporters (Got2, Slc27a1, Slc27a6, and Cd36) and for two enzymes of LCFA synthesis, stearoyl CoA desaturase-1 (Scd1), and fatty acid synthase (Fasn). Fasn and Scd1 were significantly upregulated in ob/ob and db/db mice. (b) Representative Western blots of expression of CD36 and FATP1 in control, HFD, db/db, and ob/ob mice. (c) for [3H]-oleic acid uptake in cardiomyocytes is significantly correlated with the Cd36 gene expression ratio in all obesity groups. (d) Cardiac expression ratios of 10 oxidative phosphorylation genes. Complex I: NADH dehydrogenase (Ndufaf4, Ndufa8); complex II: fumarate reductase (Sdhd); complex III: cytochrome c reductase (Cox5b, Cox6b1, and Cox6c); complex IV: cytochrome c oxidase (Uqcrc2, Uqcrfs1); Complex V: F-type ATPase (Atp5j, Atp5h) were all downregulated in all obesity groups. Error bars indicate ± 1 SE. *, ** compared with the control group.
