Effect of Inhibition of Angiotensin-Converting Enzyme and/or Neutral Endopeptidase on Neuropathy in High-Fat-Fed C57Bl/6J Mice

Table 1

Prevention study in C57Bl/6 and NEP^−/− mice. Effect of high fat diet ^+/− ilepatril, candoxatril, or enalapril on change in body weight, blood glucose, and epididymal fat mass.

Determination	Control	High fat	High fat + ilepatril	High fat + candoxatril	High fat + enalapril
C57Bl/6 mice	(12)	(11)	(12)	(12)	(10)
Start body weight (g)	27.5 ± 0.4	26.7 ± 0.5	27.2 ± 0.6	26.3 ± 0.5	27.0 ± 0.5
End body weight (g)	32.2 ± 0.6	42.9 ± 1.4^a	29.0 ± 0.9^b,c	45.3 ± 0.7^a	29.3 ± 0.5^b,c
Blood glucose (mg/dL)	188 ± 7	183 ± 8	152 ± 8	214 ± 19	177 ± 10
Epididymal fat pad (g)	0.31 ± 0.05	0.95 ± 0.08^a	0.29 ± 0.05^b,c	1.13 ± 0.08^a	0.16 ± 0.02^b,c
Fat pad/final body weight (%)	0.95 ± 0.14	2.21 ± 0.18^a	0.98 ± 0.15^b,c	2.52 ± 0.21^a	0.54 ± 0.07^b,c
NEP^−/− mice	(12)	(16)	(12)	(14)	(14)
Start body weight (g)	24.5 ± 0.6	25.6 ± 0.4	24.2 ± 0.6	25.1 ± 0.9	25.7 ± 0.5
End body weight (g)	29.1 ± 0.6	42.1 ± 1.3^a	27.1 ± 1.1^b,c	43.8 ± 2.1^a	27.3 ± 0.8^b,c
Blood glucose (mg/dL)	139 ± 8	153 ± 8	148 ± 10	173 ± 12	163 ± 6
Epididymal fat pad (g)	0.24 ± 0.03	1.09 ± 0.06^a	0.19 ± 0.02^b,c	0.90 ± 0.04^a	0.21 ± 0.06^b,c
Fat pad/final body weight (%)	0.80 ± 0.09	2.62 ± 0.13^a	0.70 ± 0.07^b,c	2.13 ± 0.15^a	0.60 ± 0.05^b,c

Data are presented as the mean ± SEM. ^aP < 0.05 compared to control; ^bP < 0.05 compared to high fat; ^cP < 0.05 compared to candoxatril. Parentheses indicate the number of experimental animals in each group.