Effect of Inhibition of Angiotensin-Converting Enzyme and/or Neutral Endopeptidase on Neuropathy in High-Fat-Fed C57Bl/6J Mice
Table 1
Prevention study in C57Bl/6 and NEP−/− mice. Effect of high fat diet +/− ilepatril, candoxatril, or enalapril on change in body weight, blood glucose, and epididymal fat mass.
Determination
Control
High fat
High fat + ilepatril
High fat + candoxatril
High fat + enalapril
C57Bl/6 mice
(12)
(11)
(12)
(12)
(10)
Start body weight (g)
27.5 ± 0.4
26.7 ± 0.5
27.2 ± 0.6
26.3 ± 0.5
27.0 ± 0.5
End body weight (g)
32.2 ± 0.6
42.9 ± 1.4a
29.0 ± 0.9b,c
45.3 ± 0.7a
29.3 ± 0.5b,c
Blood glucose (mg/dL)
188 ± 7
183 ± 8
152 ± 8
214 ± 19
177 ± 10
Epididymal fat pad (g)
0.31 ± 0.05
0.95 ± 0.08a
0.29 ± 0.05b,c
1.13 ± 0.08a
0.16 ± 0.02b,c
Fat pad/final body weight (%)
0.95 ± 0.14
2.21 ± 0.18a
0.98 ± 0.15b,c
2.52 ± 0.21a
0.54 ± 0.07b,c
NEP−/− mice
(12)
(16)
(12)
(14)
(14)
Start body weight (g)
24.5 ± 0.6
25.6 ± 0.4
24.2 ± 0.6
25.1 ± 0.9
25.7 ± 0.5
End body weight (g)
29.1 ± 0.6
42.1 ± 1.3a
27.1 ± 1.1b,c
43.8 ± 2.1a
27.3 ± 0.8b,c
Blood glucose (mg/dL)
139 ± 8
153 ± 8
148 ± 10
173 ± 12
163 ± 6
Epididymal fat pad (g)
0.24 ± 0.03
1.09 ± 0.06a
0.19 ± 0.02b,c
0.90 ± 0.04a
0.21 ± 0.06b,c
Fat pad/final body weight (%)
0.80 ± 0.09
2.62 ± 0.13a
0.70 ± 0.07b,c
2.13 ± 0.15a
0.60 ± 0.05b,c
Data are presented as the mean ± SEM. aP < 0.05 compared to control; bP < 0.05 compared to high fat; cP < 0.05 compared to candoxatril. Parentheses indicate the number of experimental animals in each group.