Effect of Inhibition of Angiotensin-Converting Enzyme and/or Neutral Endopeptidase on Neuropathy in High-Fat-Fed C57Bl/6J Mice

Table 2

Intervention study in C57Bl/6 and NEP^−/− mice. Effect of high fat diet^+/− ilepatril, candoxatril, or enalapril on change in body weight, blood glucose, and epididymal fat mass.

Determination	Control	High fat	High fat + ilepatril	High fat + candoxatril	High fat + enalapril
C57Bl/6 mice	(23)	(23)	(12)	(11)	(12)
Start Body Weight (g)	27.3 ± 0.3	26.9 ± 0.4	26.6 ± 0.3	27.9 ± 0.6	27.3 ± 0.6
12 week Weight (g)	31.8 ± 0.3	44.9 ± 0.8^a	46.4 ± 1.2^a	44.2 ± 1.4^a	44.2 ± 1.3^a
End Body Weight (g)	36.6 ± 0.4	49.1 ± 1.1^a,d	43.3 ± 1.8^a,b	52.2 ± 1.6^a,d	33.7 ± 0.9^b,d
Blood glucose (mg/dL)	170 ± 9	168 ± 11	156 ± 9	149 ± 11	192 ± 10
Epididymal fat pad (g)	0.45 ± 0.04	0.85 ± 0.05^a	1.06 ± 0.09^a	0.92 ± 0.04^a	0.57 ± 0.06^b
Fat pad/Final body; weight (%)	1.36 ± 0.10	1.75 ± 0.11^a,c	2.43 ± 0.15^a,b	1.87 ± 0.13^a	1.67 ± 0.14^c
NEP^−/− mice	(9)	(10)	(9)	(10)	(10)
Start Body Weight (g)	26.4 ± 0.4	27.6 ± 1.0	26.1 ± 1.2	26.2 ± 1.1	25.8 ± 0.4
12 week Weight (g)	28.0 ± 0.5	36.8 ± 2.1^a	34.7 ± 1.4^a	36.4 ± 1.2^a	34.8 ± 1.5^a
End Body Weight (g)	30.4 ± 0.8	50.7 ± 1.9^a,d	29.8 ± 0.8^b	50.3 ± 1.2^a,d	30.5 ± 1.0^b
Blood glucose (mg/dL)	141 ± 7	172 ± 16	173 ± 8	163 ± 10	184 ± 13
Epididymal fat pad (g)	0.23 ± 0.06	0.83 ± 0.03^a	0.45 ± 0.05^b	0.86 ± 0.04^a	0.48 ± 0.08^b
Fat pad/Final body; weight (%)	0.73 ± 0.17	1.64 ± 0.07^a	1.48 ± 0.15^a	1.71 ± 0.07^a	1.53 ± 0.19^a

Data are presented as the mean ± SEM. ^aP < 0.05 compared to control; ^bP < 0.05 compared to high fat; ^cP < 0.05 compared to ilepatril; ^dP < 0.05 compared to 12 week animals. Parentheses indicate the number of experimental animals in each group.