Catechins and Caffeine Inhibit Fat Accumulation in Mice through the Improvement of Hepatic Lipid Metabolism

Figure 3

Effects of catechins, EGCG, and caffeine on protein levels of fatty acid synthase in liver in mice. (a1) Fluorescence intensity of fatty acid synthase (FAS) by Western Blotting analysis in liver in mice fed the diets containing 0.3% catechins, 0.05% caffeine, 0.3% catechins + 0.05% caffeine for 4 weeks. Lane 1: control, lane 2: 0.3% catechins, lane 3: 0.05% caffeine, lane 4: 0.3% catechins + 0.05% caffeine. (b1) Fluorescence intensity of FAS by Western Blotting analysis in liver in mice fed the diets containing 0.1% EGCG, 0.05% caffeine, 0.1% EGCG + 0.05% caffeine for 4 weeks. Lane 1: control, lane 2: 0.1% EGCG, lane 3: 0.05% caffeine, lane 4: 0.1% EGCG + 0.05% caffeine. (a2 and b2) The protein levels of FAS determined by Western Blotting analysis in liver in mice fed the diets containing catechins and caffeine (a2) and EGCG and caffeine (b2) for 4 weeks. The level was determined from the fluorescence intensity by a image analysis system and showed as a relative value to the control. Results are given as mean ± SE of 8 mice in each groups.