Figure 6: Average immunoreactive c-Fos counts in the arcuate nucleus and orbitofrontal cortex. Immunopositive cell counts means ± SE. There was an overall increase in cell number in animals injected with nisoxetine (3 mg/kg), which is indicated by * ( ). (a) Arcuate nucleus of the hypothalamus. Planned comparisons revealed a significant effect for animals with high-fat diet exposure and nisoxetine. # indicates a difference ( ) between High Fat (Nisoxetine) and control diet (Nisoxetine). (b) Orbitofrontal cortex. There were no significant differences between diet conditions in this region.