PTPIP51: A New Interaction Partner of the Insulin Receptor and PKA in Adipose Tissue

Figure 4

Duolink proximity ligation assay to detect interactions between PTPIP51 and the insulin receptor (IR), respectively PTPIP51 and PKA in standard diet animals. (A) Interaction between PTPIP51 and the IR. (B) Interaction between PTPIP51 and PKA. (C) Negative control, PTPIP51 and the IR in testis, no interaction known. (D) Negative control, DPLA without primary antibodies in adipose tissue. Nuclei are marked by DAPI staining. Arrows: positive reactions. Bars (A, B): 50 μm. Bars (C, D): 20 μm.