Genetic and Diet-Induced Obesity Increased Intestinal Tumorigenesis in the Double Mutant Mouse Model Multiple Intestinal Neoplasia X Obese via Disturbed Glucose Regulation and Inflammation

Table 2

Associations between various parameters for body weight and glucose, insulin and cytokine levels.

	AUC bw		Terminal bw		Terminal BMI
Terminal bw	0.91	<0.001
Terminal BMI	0.59	<0.001	0.62	<0.001
Glucose; 6 weeks	0.46	<0.001	0.47	<0.001	0.57	<0.001
Glucose; 11 weeks	0.33	<0.001	0.33	<0.001	0.43	<0.001
Insulin	0.34	<0.001	0.32	<0.001	0.48	<0.001
IL-1β	0.00	n.s.	0.00	n.s.	0.01	n.s.
TNFα	0.20	0.021	0.23	0.013	0.07	n.s.

Associations between various parameters for body weight (independent variables) and glucose, insulin, and cytokine levels (dependent variables) were examined with simple linear regression (SigmaPlot 12.3, Systat Software Inc., San Jose, CA, USA). This was performed on pairs of end points from all mice from all experimental groups from which individual data could be paired. Body weight (bw) data were evaluated either as area under the curve from week 3 to 11 (AUC bw), as terminal bw, or as terminal body mass index (BMI) at 11 weeks of age. Nonfasted blood glucose was measured at 6 and 11 weeks of age. Insulin was measured with ELISA, and TNFα was measured with flow cytometer, both in plasma obtained at termination at 11 weeks. = coefficient of determination, n.s. = not statistically significant.