Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding

Figure 1

Six miRNAs whose expression changed in subcutaneous adipose tissue (SAT) three years after LAGB (T1) and Western blot evaluation of protein PPARα, which is targeted by miR-519d. (a) Expression levels of 4 downexpressed (miR-212, miR-299-5p, miR-519d, and miR-671-3p) and 2 upexpressed miRNAs (miR-370 and miR-487a) after LAGB (T1 versus T0). MiRNA expression levels are shown as mean (the miRNA expression values were first normalized to RNU48, after which the relative quantification was calculated as RQ = , where ΔΔCt = [ − ] – [ – ]. (b) Western blot evaluation of protein PPAR-α from SAT of 3 obese patients before (T0) and three years (T1) after LAGB.

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